Quantification of N-acetyl-seryl-aspartyl-lysyl-proline in hemodialysis patients administered angiotensin-converting enzyme inhibitors by stable isotope dilution liquid chromatography-tandem mass spectrometry.

@article{Inoue2011QuantificationON,
  title={Quantification of N-acetyl-seryl-aspartyl-lysyl-proline in hemodialysis patients administered angiotensin-converting enzyme inhibitors by stable isotope dilution liquid chromatography-tandem mass spectrometry.},
  author={Koichi Inoue and Ayaka Ikemura and Yoshinari Tsuruta and Kazuki Watanabe and Kaname Tsutsumiuchi and Tomoaki Hino and Hisao Oka},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2011},
  volume={54 4},
  pages={
          765-71
        }
}
We developed a sensitive, selective and accurate method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) to determine N-terminal thymosin-β peptides of Ac-SDKP and Ac-ADKP in human plasma samples. Quantification of Ac-SDKP and Ac-ADKP was performed using solid phase extraction (SPE) based on C(18), reversed phase LC separation, and stable isotope dilution electrospray ionization-MS/MS in multiple reaction-monitoring (MRM) mode. The Ac-SDKP-(13)C(6), (15)N(2) and Ac-ADKP-d… CONTINUE READING