Quantification of human urinary exosomes by nanoparticle tracking analysis.

@article{Oosthuyzen2013QuantificationOH,
  title={Quantification of human urinary exosomes by nanoparticle tracking analysis.},
  author={Wilna Oosthuyzen and Nicole E L Sime and Jessica R. Ivy and Emma J Turtle and Jonathan M. Street and John D. Pound and Louise E. Bath and David J. Webb and Christopher D Gregory and M A Bailey and J W Dear},
  journal={The Journal of physiology},
  year={2013},
  volume={591 23},
  pages={5833-42}
}
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA from the glomerulus and all sections of the nephron and represent a reservoir for biomarker discovery. Current methods for the identification and quantification of urinary exosomes are time consuming and only semi-quantitative. Nanoparticle tracking analysis (NTA) counts and sizes particles by measuring their Brownian motion in solution. In this study, we applied NTA to human urine and identified… CONTINUE READING
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Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers

  • ML Alvarez, M Khosroheidari, R Kanchi Ravi, JK DiStefano
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