Quantification of human herpesvirus 6 in healthy volunteers and patients with lymphoproliferative disorders by PCR-ELISA.

@article{Ohyashiki1999QuantificationOH,
  title={Quantification of human herpesvirus 6 in healthy volunteers and patients with lymphoproliferative disorders by PCR-ELISA.},
  author={Junko H Ohyashiki and Kenji Abe and Tomoko Ojima and P-N Wang and C F Zhou and Aya Suzuki and Kazuma Ohyashiki and Kohtaro Yamamoto},
  journal={Leukemia research},
  year={1999},
  volume={23 7},
  pages={625-30}
}
To determine whether actual numbers of human herpesvirus 6 (HHV-6) genome in hematologic neoplasias are associated with disease condition, we developed a quantitative PCR-ELISA for detection of HHV-6. The amount of viral DNA was determined using externally amplified known amounts of the plasmid DNA containing the viral target sequences. First, we determined a viral burden in peripheral blood leukocytes obtained from 23 healthy volunteers and four specimens of lymph nodes with reactive… CONTINUE READING

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