Quantification of glial fibrillary acidic protein: comparison of slot-immunobinding assays with a novel sandwich ELISA.

@article{OCallaghan1991QuantificationOG,
  title={Quantification of glial fibrillary acidic protein: comparison of slot-immunobinding assays with a novel sandwich ELISA.},
  author={James P O'Callaghan},
  journal={Neurotoxicology and teratology},
  year={1991},
  volume={13 3},
  pages={275-81}
}
Detailed protocols are presented for assaying glial fibrillary acidic protein (GFAP), an astrocyte localized protein which serves as a quantitative marker of toxicant-induced injury to the central nervous system. Two different solid-phase assay procedures are described: 1) a nitrocellulose based slot-immunobinding assay and 2) a novel microtiter plate based sandwich ELISA. The performance of both assays was assessed by measuring the content of GFAP in homogenates of specific regions of the rat… CONTINUE READING
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