Quantification of free sialic acid in human plasma through a robust quinoxalinone derivatization and LC-MS/MS using isotope-labeled standard calibration.

Abstract

We report an accurate quantification of free sialic acid (SA) in human plasma using LC-MS/MS method with isotope-labeled standard calibration (ILSC) and robust derivatization. Specifically, derivatization of SA with a stable and inexpensive 3,4-diaminotoluene (DAT) provides a stable product of SA with high MS response, proving a convenient and cost… (More)
DOI: 10.1016/j.jchromb.2013.11.016

Topics

6 Figures and Tables

Cite this paper

@article{Wang2014QuantificationOF, title={Quantification of free sialic acid in human plasma through a robust quinoxalinone derivatization and LC-MS/MS using isotope-labeled standard calibration.}, author={Dan Wang and Xiang Zhou and Lin Wang and Sihe Wang and Xue-Long Sun}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2014}, volume={944}, pages={75-81} }