Quantification of fluoxetine and norfluoxetine serum levels by reversed-phase high-performance liquid chromatography with ultraviolet detection.

@article{Holladay1997QuantificationOF,
  title={Quantification of fluoxetine and norfluoxetine serum levels by reversed-phase high-performance liquid chromatography with ultraviolet detection.},
  author={John W. Holladay and M. J. Dewey and Sun Dong Yoo},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1997},
  volume={704 1-2},
  pages={
          259-63
        }
}
A rapid and sensitive high-performance liquid chromatography assay method was developed to determine serum fluoxetine and norfluoxetine levels by single extraction of 0.1 ml of serum with sodium hydroxide. The mobile phase (55% acetonitrile-45% distilled water containing 10 mM aqueous triethylamine) was used to separate fluoxetine and norfluoxetine (25-1000 ng/ml, using clomipramine as the internal standard) by ultraviolet detection at 226 nm. The inter- and intra-day variabilities of… CONTINUE READING
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