Quantification of fibronectin adsorption to silicone-rubber cell culture substrates.

@article{Cunningham2002QuantificationOF,
  title={Quantification of fibronectin adsorption to silicone-rubber cell culture substrates.},
  author={James J. Cunningham and Janeta Nikolovski and Jennifer J. Linderman and David J Mooney},
  journal={BioTechniques},
  year={2002},
  volume={32 4},
  pages={
          876, 878, 880 passim
        }
}
As the role of mechanical force in cellular signaling gained recognition, investigators designed a number of devices to deliver controlled regimens of mechanical force to cultured cells. One type of device uses thin silicone-rubber membranes to support monolayer cell adhesion and to transmit mechanical force in the form of biaxial strain. We have observed that cell attachment and spreading are impaired on these membranes compared to polystyrene, even when both are passively coated with… CONTINUE READING

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