Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.

@article{Henry2004QuantificationOD,
  title={Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.},
  author={Sonia Henry and Ez{\'e}kiel Baudoin and Juan Carlos L{\'o}pez-Guti{\'e}rrez and Fabrice Martin-laurent and Alain Brauman and Laurent Philippot},
  journal={Journal of microbiological methods},
  year={2004},
  volume={59 3},
  pages={327-35}
}
Denitrification, the reduction of nitrate to nitrous oxide or dinitrogen, is the major biological mechanism by which fixed nitrogen returns to the atmosphere from soil and water. Microorganisms capable of denitrification are widely distributed in the environment but little is known about their abundance since quantification is performed using fastidious and time-consuming MPN-based approaches. We used real-time PCR to quantify the denitrifying nitrite reductase gene (nirK), a key enzyme of the… CONTINUE READING
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