Quantification of dendritic spine populations using image analysis and a tilting disector.

@article{Rusakov1995QuantificationOD,
  title={Quantification of dendritic spine populations using image analysis and a tilting disector.},
  author={Dmitri A. Rusakov and Michael G. Stewart},
  journal={Journal of neuroscience methods},
  year={1995},
  volume={60 1-2},
  pages={11-21}
}
A series of image analysis routines, stochastic geometry methodology, and a design-based stereological procedure have been developed to quantify objectively the length, layout, and the true density of neuronal dendritic spines observed at the light (or confocal) microscope level. First, the image of a dendritic fragment of interest (in the plane of view) is scaled to a standard brightness scale, and the dendritic profile is separated from the background using a computerized thresholding… CONTINUE READING
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