Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.

@article{Hardwick2008QuantificationOC,
  title={Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.},
  author={Simon A. Hardwick and H. W. Stokes and Sophia Findlay and Mark Taylor and Michael R. Gillings},
  journal={FEMS microbiology letters},
  year={2008},
  volume={278 2},
  pages={207-12}
}
Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in clinical environments. However, virtually nothing is known about the distribution or abundance of class 1 integrons outside of the clinical context. Here we develop a SYBR Green… CONTINUE READING