Quantification of chlordesmethyldiazepam by liquid chromatography-tandem mass spectrometry: application to a cloxazolam bioequivalence study.

@article{OliveiraSilva2009QuantificationOC,
  title={Quantification of chlordesmethyldiazepam by liquid chromatography-tandem mass spectrometry: application to a cloxazolam bioequivalence study.},
  author={Diogo Oliveira-Silva and C. H. Oliveira and G. Mendes and P. Galvinas and R. Barrientos-Astigarraga and G. De Nucci},
  journal={Biomedical chromatography : BMC},
  year={2009},
  volume={23 12},
  pages={
          1266-75
        }
}
A rapid, sensitive and specific LC-MS/MS method was developed and validated for quantifying chlordesmethyldiazepam (CDDZ or delorazepam), the active metabolite of cloxazolam, in human plasma. In the analytical assay, bromazepam (internal standard) and CDDZ were extracted using a liquid-liquid extraction (diethyl-ether/hexane, 80/20, v/v) procedure. The LC-MS/MS method on a RP-C18 column had an overall run time of 5.0 min and was linear (1/x weighted) over the range 0.5-50 ng/mL (R > 0.999). The… Expand
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