Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain

@article{Wilcock2006QuantificationOC,
  title={Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain},
  author={Donna M Wilcock and Marcia N Gordon and David Morgan},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={1591-1595}
}
In the current protocol, we describe the Congo red staining method and a method for separately quantifying vascular and parenchymal amyloid deposits in brain tissue sections. Congo red staining detects amyloid deposits in brain tissue of amyloid precursor protein transgenic mice and human Alzheimer's tissue. It detects compacted amyloid in a β-sheet secondary structure and labels amyloid in both the brain parenchyma (amyloid plaques) and blood vessels. Congophilic amyloid in blood vessels is… CONTINUE READING

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