Quantification of carbamylated LDL in human sera by a new sandwich ELISA.

@article{Apostolov2005QuantificationOC,
  title={Quantification of carbamylated LDL in human sera by a new sandwich ELISA.},
  author={Eugene O. Apostolov and Sudhir V. Shah and Ercan Ok and Alexei G. Basnakian},
  journal={Clinical chemistry},
  year={2005},
  volume={51 4},
  pages={
          719-28
        }
}
BACKGROUND We previously suggested that increased carbamylated LDL (cLDL), a product of nonenzymatic modification of LDL in human serum by urea-derived cyanate, may cause cardiovascular complications in patients with chronic renal insufficiency. An assay for precise measurement of cLDL in serum was not previously available. METHODS Polyclonal antibodies against human cLDL and nonmodified, native LDL (nLDL) were raised in rabbits and extensively purified by affinity chromatography. New… 
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ATR-FTIR spectroscopy can be considered as a novel method to detect non- HDL carbamoylation on spectral changes of non-high-density lipoproteins (non-HDL) in chronic kidney disease patients.
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TLDR
The present results raise the possibility that HDL carbamylation contributes to foam cell formation in atherosclerotic lesions through a pathway requiring the HDL-receptor scavenger receptor class B, type I.
Measurement of Homocitrulline, A Carbamylation‐derived Product, in Serum and Tissues by LC‐MS/MS
TLDR
This unit describes a liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the quantification of homocitrulline, with special emphasis on pre‐analytical steps that allow quantifying of total or protein‐bound homocitsine in serum or tissue samples.
Chronic uremia stimulates LDL carbamylation and atherosclerosis.
TLDR
Elevated plasma cLDL may represent an independent risk factor for uremia-induced atherosclerosis and is demonstrated in surgically induced CKD in apolipoprotein E-deficient mice fed a high-fat diet to promote the development of Atherosclerosis.
Carbamylated Low-Density Lipoproteins Induce a Prothrombotic State Via LOX-1: Impact on Arterial Thrombus Formation In Vivo.
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