Quantification of bands produced by isoelectric focusing using immunoperoxidase.

@article{Paluch1984QuantificationOB,
  title={Quantification of bands produced by isoelectric focusing using immunoperoxidase.},
  author={U H Paluch and Geoffrey Keir and Sheila Moyle and Edward J. Thompson},
  journal={Journal of clinical pathology},
  year={1984},
  volume={37 10},
  pages={1172-6}
}
An improved, rapid, and sensitive (to autoradiographic levels) method for the quantification of IgG bands from cerebrospinal fluid is presented. The principle of an internal standard is employed using the discrete bands of a paraprotein to correct for any run to run variability in the enzyme detection system which is linked to the immunoassay of immobilised antigen.