Quantification of bacterial polyhydroxyalkanoic acids by Nile red staining

Abstract

The fluorescence properties of one chemically and seven biologically produced polyhydroxyalkanoic acids were investigated as film castings and in living cells respectively after staining with Nile red. All these polyesters show a similar fluorescence behaviour, revealing a clear fluorescence maximum at an excitation wavelength between 540 nm and 560 nm and… (More)
DOI: 10.1007/s002530051460

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