Quantification of aquatic viruses by flow cytometry
@inproceedings{Brussaard2016QuantificationOA, title={Quantification of aquatic viruses by flow cytometry}, author={Corina P. D. Brussaard}, year={2016} }
Abstract For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic sys-tems because of its high reproducibility, high sample throughput, and ability to distinguish several subpopula-tions of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typicallyshows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry.Here we describe in detail all steps in the…
101 Citations
Flow cytometric enumeration of marine viral populations at low abundances
- Environmental Science
- 2014
Raising the pH of the Tris-EDTA (TE) buffer to 8.2 successfully countered the effect of insufficient buffering capacity at low dilutions, which is caused by the higher proportion of acidic glutaraldehyde fixative in the final sample.
An Inexpensive, Accurate, and Precise Wet-Mount Method for Enumerating Aquatic Viruses
- Environmental ScienceApplied and Environmental Microbiology
- 2015
The wet-mount method is significantly less expensive than the filter mount method and is appropriate for rapid, precise, and accurate enumeration of aquatic viruses over a wide range of viral concentrations encountered in field and laboratory samples.
Visualization of Viral Infection Dynamics in a Unicellular Eukaryote and Quantification of Viral Production Using Virus Fluorescence in situ Hybridization
- BiologyFrontiers in Microbiology
- 2020
VirusFISH is demonstrated to be a promising technique to study specific virus-host interactions in non-axenic cultures and establish a framework for its application in complex natural communities.
HOST MICROBE INTERACTIONS
- Environmental Science, Biology
- 2020
The results demonstrate that the relative abundance of HNA and LNA heterotrophic cells andheterotrophic prokaryote production vary according to organic carbon variability in the water column, and suggest groupand activity-specific linkages between hosts and viruses on the flux of carbon through the microbial food web.
Deciphering the Virus Signal Within the Marine Dissolved Organic Matter Pool
- Environmental ScienceFrontiers in Microbiology
- 2022
Viruses are ubiquitously distributed in the marine environment, influencing microbial population dynamics and biogeochemical cycles on a large scale. Due to their small size, they fall into the…
Simultaneous Giant Virus and Virophage Quantification Using Droplet Digital PCR
- BiologyViruses
- 2022
The results show that ddPCR can be an efficient method to quantify virus and virophage, and potential applications of the method for studying ecological and evolutionary processes of virus andvirophages are discussed.
Regulation of Low and High Nucleic Acid Fluorescent Heterotrophic Prokaryote Subpopulations and Links to Viral-Induced Mortality Within Natural Prokaryote-Virus Communities
- Environmental Science, BiologyMicrobial Ecology
- 2019
The results demonstrate that the relative abundance of HNA and LNA heterotrophic cells andheterotrophic prokaryote production vary according to organic carbon variability in the water column, which can be strongly influenced by the physical eddy field.
Abundance and observations of thermophilic microbial and viral communities in submarine and terrestrial hot fluid systems of the French Southern and Antarctic Lands
- Environmental SciencePolar Biology
- 2018
A positive correlation was found between VLP and prokaryotic abundances, while the virus-to-prokaryote ratio was generally low and ranged between 0.1 and 6.1 when FCM was applied.
Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples
- Environmental SciencePloS one
- 2012
Sodium azide cannot be used to maintain marine samples used for the enumeration of prokaryotes and viruses and the potential of sodium azide as a preservative as a possible alternative is tested.
References
SHOWING 1-10 OF 39 REFERENCES
Optimization of Procedures for Counting Viruses by Flow Cytometry
- Environmental ScienceApplied and Environmental Microbiology
- 2004
The results from examinations of storage conditions clearly demonstrated the importance of low storage temperatures to prevent strong decreases in measured total virus abundance with time.
Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry
- Environmental ScienceApplied and Environmental Microbiology
- 1999
Flow cytometry (FCM) was successfully used to enumerate viruses in seawater after staining with the nucleic acid-specific dye SYBR Green-I, and it revealed that the abundance of viruses displayed the same vertical trend as that of planktonic cells.
Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy
- Environmental Science
- 1995
The results indicate that the concentration of viruses in natural waters may be higher than previously recognized and imply that the TEM-based method significantly underestimates virus abundance.
Population dynamics and diversity of phytoplankton, bacteria and viruses in a seawater enclosure
- Environmental Science
- 2001
A close linkage between various algal, bacterial and viral populations is indicated and it is shown that virioplankton do not necessarily termi- nate algal and bacterial blooms but that they keep the host populations at non-blooming levels.
Comparison of flow cytometry and epifluorescence microscopy for counting bacteria in aquatic ecosystems.
- Biology, MedicineCytometry
- 1992
The results of this study showed that flow cytometry was a reliable technique for counting a mixture of bacteria in samples from aquatic ecosystems.
Monitoring phytoplankton, bacterioplankton, and virioplankton in a coastal inlet (Bedford Basin) by flow cytometry.
- Environmental ScienceCytometry
- 2001
The microbial biomass in the surface water of Bedford Basin is dominated by phytoplankton, and a vigorous microbial loop and viral shunt is envisioned to occur in the summer.
Temporal distribution of viruses, bacteria and phytoplankton throughout the water column in a freshwater hypereutrophic lake
- Environmental Science
- 2005
Analytical flow cytometry and denaturing gradient gel electrophoresis were used to investigate the diversity and dynamics of viruses, bacteria and phytoplankton in a hypereutrophic freshwater lake and appeared to be closely linked to other biotic and abiotic parameters.
Determination of Virus Abundance in Marine Sediments
- Environmental ScienceApplied and Environmental Microbiology
- 2001
This study optimized procedures to enumerate viruses from marine sediments by epifluorescence microscopy using SYBR Green I as a stain using pyrophosphate-ultrasound treatment, suggesting a masking effect of extracellular DNA.
Application of Digital Image Analysis and Flow Cytometry To Enumerate Marine Viruses Stained with SYBR Gold
- BiologyApplied and Environmental Microbiology
- 2001
A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples and yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry.