Quantification of an Eikelboom type 021N bulking event with fluorescence in situ hybridization and real-time PCR

  title={Quantification of an Eikelboom type 021N bulking event with fluorescence in situ hybridization and real-time PCR},
  author={Han Vervaeren and Kurt De Wilde and Jorg Matthys and Nico Boon and Lutgarde M. Raskin and Willy Verstraete},
  journal={Applied Microbiology and Biotechnology},
Primers targeting 16S rRNA genes were designed to detect and quantify Eikelboom type 021N organisms by real-time PCR. Eikelboom type 021N filamentous bulking was induced in a laboratory-scale sequencing batch reactor and the evolution of Eikelboom type 021N 16S rRNA and 16S rRNA genes was monitored. A significant correlation was found between the sludge volume index and the amount of these filamentous organisms present in the sludge (r 2=94.6%, n=10, P<0.01), as measured by real-time PCR. The… CONTINUE READING
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