Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR.

  title={Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR.},
  author={A Hermansson and Per Eric Lindgren},
  journal={Applied and environmental microbiology},
  volume={67 2},
Real-time PCR was used to quantify populations of ammonia-oxidizing bacteria representing the beta subdivision of the class Proteobacteria in samples of arable soil, both nitrogen fertilized and unfertilized, from Mellby, Sweden. Primers and probes targeting a 16S ribosomal DNA region of the ammonia-oxidizing bacteria were designed and used. In the fertilized soil there were approximately 6.2 x 10(7) ammonia-oxidizing bacteria per g of soil, three times more than the number of bacteria in the… CONTINUE READING


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Environmental factors controlling NO32 leaching, N2O emissions and numbers of NH41 oxidisers in a coniferous forest soil

  • M. Carnol, P. Ineson
  • Soil Biol. Biochem
  • 1999
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