Quantification of alkylresorcinol metabolites in urine by HPLC with coulometric electrode array detection.

  title={Quantification of alkylresorcinol metabolites in urine by HPLC with coulometric electrode array detection.},
  author={Anja H Koskela and Anna-Maria Linko-Parvinen and Perttu Hiisivuori and Adile Samaletdin and Afaf Kamal-Eldin and Matti J. Tikkanen and Herman C T Adlercreutz},
  journal={Clinical chemistry},
  volume={53 7},
BACKGROUND Whole-grain rye and wheat cereals contain high amounts of alkylresorcinols (ARs), phenolic lipids. ARs can be quantified in plasma. Two recently identified urinary AR metabolites, 3,5-dihydroxyphenylbenzoic acid (DHBA) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA), may be useful as biomarkers of intake of whole-grain rye and wheat. METHODS We evaluated 4 pretreatment protocols for quantifying urinary DHBA and DHPPA using HPLC coupled with a coulometric electrode array… CONTINUE READING
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