Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

@article{Vermere2012QuantificationOR,
  title={Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors},
  author={Jolien Verme{\'i}re and Evelien Naessens and Hanne Vanderstraeten and Alessia Landi and Veronica Iannucci and Anouk van Nuffel and Tom Taghon and Massimo Pizzato and Bruno Verhasselt},
  journal={PLoS ONE},
  year={2012},
  volume={7},
  pages={263 - 267}
}
  • Jolien Vermeíre, Evelien Naessens, +6 authors Bruno Verhasselt
  • Published in PloS one 2012
  • Medicine, Biology
  • PLoS ONE
  • Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dynamic range of detection. In contrast, measurement of reverse transcriptase (RT) activity is a generic method which can be adapted for higher sensitivity using real-time PCR quantification (qPCR-based… CONTINUE READING

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