Quantification of NGF-dependent neuronal differentiation of PC-12 cells by means of neurofilament-L mRNA expression and neuronal outgrowth.

@article{Schimmelpfeng2004QuantificationON,
  title={Quantification of NGF-dependent neuronal differentiation of PC-12 cells by means of neurofilament-L mRNA expression and neuronal outgrowth.},
  author={Jutta Schimmelpfeng and K. F. Weibezahn and Hermann F. Dertinger},
  journal={Journal of neuroscience methods},
  year={2004},
  volume={139 2},
  pages={299-306}
}
We demonstrate that the degree of neuronal development of PC-12 cell differentiation can be quantified by the expression of neurofilament-L (NF-L) mRNA, when an optimal concentration of NGF (50 ng/ml) is used. During the first 7 days of NGF treatment, the relative amount of NF-L mRNA was found to increase continuously and to correlate with the outgrowth of neurites in a statistically significant way. Thus, mRNA expression is, under these conditions, a suitable means for reliably monitoring the… CONTINUE READING
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