Quantification of Ebola virus replication kinetics in vitro

@article{Liao2020QuantificationOE,
  title={Quantification of Ebola virus replication kinetics in vitro},
  author={Laura E. Liao and Jonathan Carruthers and Sophie Smither and Simon A Weller and Diane Williamson and Thomas R. Laws and Isabel Garc{\'i}a-Dorival and Julian A. Hiscox and Benjamin P. Holder and Catherine A. A. Beauchemin and Alan S. Perelson and Mart{\'i}n L{\'o}pez-Garc{\'i}a and Grant D. Lythe and John N. Barr and Carmen Molina-Par{\'i}s},
  journal={PLoS Computational Biology},
  year={2020},
  volume={16}
}
Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities for experimentation, modelling can play a crucial role. Ebola virus modelling efforts have primarily focused on in vivo virus kinetics, e.g., in animal models, to aid the development of antivirals and vaccines. But, thus far, these studies have not yielded a detailed specification of the infection cycle, which could… 

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References

SHOWING 1-10 OF 61 REFERENCES
Ebola virus infection modeling and identifiability problems
TLDR
A first mathematical approach of EBOV dynamics and the estimation of standard parameters in viral infections kinetics is the key contribution of this work, paving the way for future modeling works on E BOV infection.
Quantification system for the viral dynamics of a highly pathogenic simian/human immunodeficiency virus based on an in vitro experiment and a mathematical model
TLDR
A new mathematical model is introduced which tracks both infectious and total viral load, as well as the fraction of infected and uninfected cells within a cell culture, and is applied to analyze time-course data of an SHIV infection in vitro to significantly improve the understanding of SHIV and HIV-1 pathogenesis.
Modelling Ebola virus dynamics: Implications for therapy.
Modeling Challenges of Ebola Virus–Host Dynamics during Infection and Treatment
TLDR
The observations of EBOV kinetics in multiple anatomic compartments and hepatocellular injury in a critically ill patient with Ebola virus disease (EVD) are compared and the model’s predictions are explored, with and without antiviral therapy.
Kinetics of Influenza A Virus Infection in Humans
TLDR
A series of mathematical models of increasing complexity, which incorporate target cell limitation and the innate interferon response, are utilized to examine influenza A virus kinetics in the upper respiratory tracts of experimentally infected adults to suggest that antiviral treatments have a large hurdle to overcome in moderating symptoms and limiting infectiousness.
Ebola virus dynamics in mice treated with favipiravir.
Ebola viral dynamics in nonhuman primates provides insights into virus immuno-pathogenesis and antiviral strategies
TLDR
An integrative mathematical model is used to correlate the dose and the time of treatment initiation with survival rate, enhanced immune response and viral clearance and can help optimize antiviral evaluation in future outbreaks of Ebola.
Uncovering critical properties of the human respiratory syncytial virus by combining in vitro assays and in silico analyses
TLDR
It is found that RSV spreads inefficiently in SIAT cells, in a manner that is consistent with the establishment of infection resistance in uninfected cells, and highlights the importance and urgency of resolving the mechanisms at play in the dissemination of RSV infections in vitro.
Duration of SHIV production by infected cells is not exponentially distributed: Implications for estimates of infection parameters and antiviral efficacy
TLDR
It is shown that incorrect assumptions about the eclipse and virus-producing phase distributions can lead to an overestimation of antiviral efficacy, and predictions for the rate of plasma HIV decay under integrase inhibitor therapy offer an opportunity to confirm whether HIV production duration in vivo also follows a normal distribution, as demonstrated here for SHIV infections in vitro.
Zika plasma viral dynamics in nonhuman primates provides insights into early infection and antiviral strategies
TLDR
Using mathematical modeling, it is found that, in nonhuman primates, the viral dynamics of ZIKV are characterized by a short lifetime of infected cells (<10 h) during which enough viral particles are produced to infect ∼11 other cells, and higher disease burden is associated with changes in natural killer cell subset concentrations.
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