Quantification of Detergents Complexed with Membrane Proteins

  title={Quantification of Detergents Complexed with Membrane Proteins},
  author={Vincent Chaptal and Fr{\'e}d{\'e}ric Delolme and Arnaud Kilburg and Sandrine Magnard and C{\'e}dric Montigny and Martin Picard and Charl{\`e}ne Prier and Luca Monticelli and Olivier Bornert and Morgane Agez and St{\'e}phanie Ravaud and C{\'e}dric Orelle and R. Wagner and Anass Jawhari and Isabelle Broutin and Eva Pebay-Peyroula and Jean-Michel Jault and H Ronald Kaback and Marc le Maire and Pierre Guy Falson},
  booktitle={Scientific reports},
Most membrane proteins studies require the use of detergents, but because of the lack of a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional and structural data analyses. To solve this problem, we propose a method based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) that allows quantification of pure or mixed detergents in complex with membrane proteins. We validated the method with a wide variety of… CONTINUE READING
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