Quantification of DNaseI-sensitivity by real-time PCR: quantitative analysis of DNaseI-hypersensitivity of the mouse beta-globin LCR.

@article{McArthur2001QuantificationOD,
  title={Quantification of DNaseI-sensitivity by real-time PCR: quantitative analysis of DNaseI-hypersensitivity of the mouse beta-globin LCR.},
  author={Maggie McArthur and Sabine Gerum and G. Stamatoyannopoulos},
  journal={Journal of molecular biology},
  year={2001},
  volume={313 1},
  pages={27-34}
}
We employ real-time PCR to allow us to quantify the sensitivity of chromatin to digestion by DNaseI. This approach has three clear advantages over the more conventional use of the Southern hybridization assay: the accuracy of quantification is improved; the resolution of the assay is enhanced, by designing primers to amplify small amplicons it is possible to analyze sequences both co-incident and proximal to sites of DNaseI-hypersensitivity; less material is needed, as little as 5 ng of treated… CONTINUE READING
Highly Influential
This paper has highly influenced 11 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
59 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 59 extracted citations

Similar Papers

Loading similar papers…