Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR.

@article{Wolffs2005QuantificationOC,
  title={Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR.},
  author={Petra T. Wolffs and B{\"o}rje Norling and Jeffrey Hoorfar and Mansel Griffiths and Peter R{\aa}dstr{\"o}m},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 10},
  pages={5759-64}
}
Real-time PCR is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish between DNA originating from viable, viable nonculturable (VNC), and dead cells. For this reason, real-time PCR has been combined with a novel discontinuous buoyant density gradient method, called flotation, in order to allow detection of only viable and VNC cells of thermotolerant campylobacters in… CONTINUE READING
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