Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR.

@article{Penders2005QuantificationOB,
  title={Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR.},
  author={John Penders and Cornelis Vink and Christel Driessen and Nancy M London and Carel Thijs and Ellen E. Stobberingh},
  journal={FEMS microbiology letters},
  year={2005},
  volume={243 1},
  pages={141-7}
}
To determine the influence of either exclusive breast-feeding or formula feeding on both composition and quantity of the gut microbiota in infants, we have developed real-time, quantitative PCR assays for the detection of Bifidobacterium spp. and Clostridium difficile. Furthermore, we have monitored the prevalence and counts of Escherichia coli by applying a previously described real-time PCR assay. We found all 100 infants tested to be colonized by Bifidobacterium spp. The bifidobacterial… CONTINUE READING
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