Quantification of Aquaporin-CHIP water channel protein in microdissected renal tubules by fluorescence-based ELISA.

@article{Maeda1995QuantificationOA,
  title={Quantification of Aquaporin-CHIP water channel protein in microdissected renal tubules by fluorescence-based ELISA.},
  author={Yorio Maeda and Barbara L Smith and Peter Agre and Mark A. Knepper},
  journal={The Journal of clinical investigation},
  year={1995},
  volume={95 1},
  pages={422-8}
}
Several transporters have been localized along the nephron by physiological methods or immunocytochemistry. However, the actual abundance of these molecules has not been established. To accomplish this goal, we have developed a fluorescence-based ELISA method and have used it to quantitate Aquaporin-CHIP (AQP-CHIP) water channel protein in rat kidney tubules. Microdissected tubules (2 mm/sample, permeabilized with 0.5% Triton X-100) or purified AQP-CHIP standards (0-200 fmol) were utilized in a… CONTINUE READING

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