Quantification of Anti-Aggregation Activity of Chaperones: A Test-System Based on Dithiothreitol-Induced Aggregation of Bovine Serum Albumin

@inproceedings{Borzova2013QuantificationOA,
  title={Quantification of Anti-Aggregation Activity of Chaperones: A Test-System Based on Dithiothreitol-Induced Aggregation of Bovine Serum Albumin},
  author={Vera A. Borzova and Kira A. Markossian and Dmitriy A. Kara and Natalia A. Chebotareva and Valentina F. Makeeva and Nikolay B. Poliansky and Konstantin Muranov and Boris I. Kurganov},
  booktitle={PloS one},
  year={2013}
}
The methodology for quantification of the anti-aggregation activity of protein and chemical chaperones has been elaborated. The applicability of this methodology was demonstrated using a test-system based on dithiothreitol-induced aggregation of bovine serum albumin at 45°C as an example. Methods for calculating the initial rate of bovine serum albumin aggregation (v agg) have been discussed. The comparison of the dependences of v agg on concentrations of intact and cross-linked α-crystallin… CONTINUE READING

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