Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating

@inproceedings{Karava2019QuantificationAI,
  title={Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating},
  author={Marianna Karava and Felix Bracharz and Johannes Kabisch},
  booktitle={PloS one},
  year={2019}
}
The Gram-positive bacterium Bacillus subtilis is able to form endospores which have a variety of biotechnological applications. Due to this ability, B. subtilis is as well a model organism for cellular differentiation processes. Sporulating cultures of B. subtilis form sub-populations which include vegetative cells, sporulating cells and spores. In order to readily and rapidly quantify spore formation we employed flow cytometric and fluorescence activated cell sorting techniques in combination… CONTINUE READING