Quantification and Localization of Bacteria in Plant Tissues Using Quantitative Real-Time PCR and Online Emission Fingerprinting

@article{Ruppel2006QuantificationAL,
  title={Quantification and Localization of Bacteria in Plant Tissues Using Quantitative Real-Time PCR and Online Emission Fingerprinting},
  author={Silke Ruppel and J{\"o}rg R{\"u}hlmann and Wolfgang Merbach},
  journal={Plant and Soil},
  year={2006},
  volume={286},
  pages={21-35}
}
In order to quantify and localize specific bacterial target genes in plant tissue, this project has generated relevant new insights in the combined application of quantitative real-time PCR in parallel with the in situ PCR + probe-hybridization and online emission fingerprinting using LSM 510 META. After designing an Enterobacter radicincitans species-specific probe, introduced bacterial cells were monitored in growing plant parts and their colonization behaviour was examined in relation to the… CONTINUE READING

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