Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology.

@article{Weis2007QualityCF,
  title={Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology.},
  author={Serge Weis and Ida C. Llenos and Jeanette R Dulay and Michael Elashoff and Francisco Mart{\'i}nez-Murillo and Christine L Miller},
  journal={Journal of neuroscience methods},
  year={2007},
  volume={165 2},
  pages={198-209}
}
The quality of results from microarray studies depends on RNA quality, which can be significantly influenced by postmortem factors. The aim of this study was to determine which postmortem factors and/or RNA electropherogram characteristics best correspond to microarray output and can be used to prospectively screen RNA prior to microarray analysis. Total RNA was extracted (N=125) from gray and white matter of postmortem frontal and occipital lobe tissue, acquired from normal controls, and… CONTINUE READING