Qualitative and quantitative analysis of hyaluronan oligosaccharides with high performance thin layer chromatography using reagent-free derivatization on amino-modified silica and electrospray ionization-quadrupole time-of-flight mass spectrometry coupling on normal phase.

@article{Rothenhfer2012QualitativeAQ,
  title={Qualitative and quantitative analysis of hyaluronan oligosaccharides with high performance thin layer chromatography using reagent-free derivatization on amino-modified silica and electrospray ionization-quadrupole time-of-flight mass spectrometry coupling on normal phase.},
  author={M. Rothenh{\"o}fer and R. Scher{\"u}bl and G. Bernhardt and J. Heilmann and A. Buschauer},
  journal={Journal of chromatography. A},
  year={2012},
  volume={1248},
  pages={
          169-77
        }
}
  • M. Rothenhöfer, R. Scherübl, +2 authors A. Buschauer
  • Published 2012
  • Chemistry, Medicine
  • Journal of chromatography. A
  • Purified oligomers of hyalobiuronic acid are indispensable tools to elucidate the physiological and pathophysiological role of hyaluronan degradation by various hyaluronidase isoenzymes. Therefore, we established and validated a novel sensitive, convenient, rapid, and cost-effective high performance thin layer chromatography (HPTLC) method for the qualitative and quantitative analysis of small saturated hyaluronan oligosaccharides consisting of 2-4 hyalobiuronic acid moieties. The use of amino… CONTINUE READING
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