Qualitative and quantitative analysis of flavonoids in the leaves of Isatis indigatica Fort. by ultra-performance liquid chromatography with PDA and electrospray ionization tandem mass spectrometry detection.

@article{Deng2008QualitativeAQ,
  title={Qualitative and quantitative analysis of flavonoids in the leaves of Isatis indigatica Fort. by ultra-performance liquid chromatography with PDA and electrospray ionization tandem mass spectrometry detection.},
  author={Xiangyu Deng and Guihua Gao and Shuning Zheng and Famei Li},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2008},
  volume={48 3},
  pages={
          562-7
        }
}
A novel method based on ultra-performance liquid chromatography with photo diode array and electrospray ionization tandem mass spectrometry detection (UPLC-PDA-ESI-MS/MS) was developed for the qualitative and quantitative analysis of flavonoids in the leaves of Isatis indigotica Fort. (Daqingye). The separation was carried out on an Acquity UPLC BEH C(18) column with 0.1% formic acid and methanol as the mobile phase under gradient conditions. Eight flavone C-glucosides were identified and their… CONTINUE READING
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