Qualitative and Quantitative Aspects of the Inheritance of Chemical Phenotype in Cannabis

@article{Mandolino2003QualitativeAQ,
  title={Qualitative and Quantitative Aspects of the Inheritance of Chemical Phenotype in Cannabis},
  author={Giuseppe Mandolino and Manuela Bagatta and A. Carboni and Paolo Ranalli and Etienne P M de Meijer},
  journal={Journal of Industrial Hemp},
  year={2003},
  volume={8},
  pages={51 - 72}
}
Abstract Four crosses were made between Cannabis plants with “pure” CBD and THC chemotypes. The F1 plants obtained were self-fertilised to produce F2s. Chemotypical distributions were analysed by gas-chromatography. A segregation analysis of the different F2 progenies obtained showed that chemotype, estimated as CBD/THC ratio, behaves as a qualitative character, and a model for a single locus B, with two co-dominant alleles, B D and B T is described. The CBD/THC ratios in the F1 offsprings were… Expand
View on Taylor & Francis
32 Citations
Background Citations
15
Methods Citations
1
Results Citations
1

32 Citations

Citation Type

Citation Type

A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae).
TLDR
A two-species concept of Cannabis is supported by the determined frequencies of B(D) and B(T) in sample populations of 157 Cannabis accessions determined from CBD and THC banding patterns, visualized by starch gel electrophoresis. Expand
Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L.
TLDR
High‐throughput PACE (PCR Allele Competitive Extension) assays for C. sativa plant sex and cannabinoid chemotype revealed segregation in hemp populations, and resolved plants producing predominantly THC, predominantly CBD, and roughly equal amounts of THC and CBD. Expand
Time course of cannabinoid accumulation and chemotype development during the growth of Cannabis sativa L
TLDR
It was confirmed that callus cultures of Cannabis were not able to produce detectable amounts of any cannabinoids, irrespective of the type and amount of cannabinoids in the mother plants. Expand
Genetics and Marker-assisted Selection of the Chemotype in Cannabis sativa L.
TLDR
The effectiveness of two different types of markers associated to the locus determining the chemotype in Cannabis was evaluated and discussed, as possible tools in marker-assisted selection in hemp, but also for possible applications in the forensic and pharmaceutical fields. Expand
A chemotaxonomic analysis of terpenoid variation in Cannabis
To determine whether the terpenoid composition of the essential oil of Cannabis is useful for chemotaxonomic discrimination, extracts of pistillate inflorescences of 162 greenhouse-grown plants of… Expand
Classification of Cannabis Plants Grown in Northern Thailand using Physico-Chemical Properties
TLDR
Stepwise discriminant analysis confirmed that cannabinoids and some physical properties could be used to classify the phenotype of cannabis plants into drug, intermediate and fiber types as well, whereas the accessions of cannabis could not be discriminated clearly by using only their physico-chemical parameters. Expand
Analysis of Sequence Variability and Transcriptional Profile of Cannabinoid synthase Genes in Cannabis sativa L. Chemotypes with a Focus on Cannabichromenic acid synthase
TLDR
The low but stable transcriptional level of CBCAS in all genotypes suggests that these genes are active and might contribute to the final amount of cannabinoids. Expand
Sequence heterogeneity of cannabidiolic- and tetrahydrocannabinolic acid-synthase in Cannabis sativa L. and its relationship with chemical phenotype.
TLDR
CBDAS is considered as the ancestral type of THCA- and CBDA-synthases because the amount of variation was found to be higher within the CBDAS sequence family than in the THCAS family, suggesting a more recent evolution of TH CA-forming enzymes from the CBDAs group. Expand
Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes.
TLDR
Differences in the cannabigerolic acid development among the different chemotypes and between monoterpene and sesquiterpene evolution patterns were also observed. Expand
Identification of Chemotypic Markers in Three Chemotype Categories of Cannabis Using Secondary Metabolites Profiled in Inflorescences, Leaves, Stem Bark, and Roots
TLDR
This study investigated the utility of the full spectrum of secondary metabolites in different plant parts in three cannabis chemotypes (THC dominant, intermediate, and CBD dominant) for chemotaxonomic discrimination and provides a comprehensive profile of bioactive compounds in three chemotypes for medical purposes. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 46 REFERENCES
The inheritance of chemical phenotype in Cannabis sativa L.
TLDR
Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes, and it is suggested that codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC. Expand
Identification of a new chemotype in Cannabis sativa: cannabigerol-dominant plants, biogenetic and agronomic prospects.
TLDR
The present paper provides the description of a highly interesting, new, “fourth“ chemotype, whose very low content in Δ9-tetrahydrocannabinol (Δ9-THC) (0.001%) in contrast with the high amounts which occur in cannabigerol (CBG), its major cannabinoīd component. Expand
Genetic diversity of Cannabis sativa germplasm based on RAPD markers
TLDR
RAPD markers provide a powerful tool for the investigation of genetic variation in cultivars/accessions of hemp and Variability within the two cultivars ‘Carmagnola’ and ‘Fibranova1’ was shown, suggesting good possibilities for long–term selection work. Expand
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
TLDR
A 400-bp RAPD marker generated by a primer of random decamer sequence has been found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp and is suitable for a precise, early and rapid identification of male plants during breeding programs of dIOecious and monoecious hemp. Expand
Characterisation of Cannabis accessions with regard to cannabinoid content in relation to other plant characters
TLDR
Variation within populations for cannabinoid content, and consistency of chemical characters at the population level were investigated and various combinations of cannabinoid content and other economic plant characters were observed, thus a breeding programme will not be hampered by strict linkage. Expand
Comparison of Hemp Varieties Using Random Amplified Polymorphic DNA Markers
TLDR
The correlations among the molecular data and the genetic structure of the different cultivars and the consequences in relation to variety discrimination in hemp are discussed. Expand
The Applications of Molecular Markers in Genetics and Breeding of Hemp
TLDR
The state of the art of sex genetics in hemp and the possibilities of developing molecular markers linked to different hemp chemotypes are discussed. Expand
Purification and Characterization of Cannabidiolic-acid Synthase from Cannabis sativa L.
TLDR
It is concluded that CBDA is predominantly biosynthesized from cannabigerolic acid rather than cannabinerolic acid, although the kcat for the former is lower than that for the latter, and that the enzymatic cyclization does not proceed via oxygenated intermediates. Expand
Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.
TLDR
Bulk segregant analysis has several advantages over the use of near-isogenic lines to identify markers in specific regions of the genome and will have widespread application both in those species where selfing is possible and in those that are obligatorily outbreeding. Expand
Biosynthesis of cannabinoids
The biosynthesis of cannabinoids was studied in cut sprouts of Cannabis sativa by incorporation experiments using mixtures of unlabeled glucose and [1-13C]glucose or [U-13C6]glucose. 13C-labeling… Expand
...
1
2
3
4
5
...