Quadruple labeling of brain-stem neurons: a multiple retrograde fluorescent tracer study of axonal collateralization.

@article{Akintunde1992QuadrupleLO,
  title={Quadruple labeling of brain-stem neurons: a multiple retrograde fluorescent tracer study of axonal collateralization.},
  author={A Akintunde and Douglas F Buxton},
  journal={Journal of neuroscience methods},
  year={1992},
  volume={45 1-2},
  pages={15-22}
}
Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. Some, though by no means all, of these axons were stem axons with terminals in 2, 3 or 4 of the injected spinal segments. Hence as many as 4 different fluorescent tracers could be… CONTINUE READING