Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus.

@article{Liu2006QuadrupleAE,
  title={Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus.},
  author={Siguo Liu and Xinglong Yu and Chunlai Wang and Jianmin Wu and Xiangang Kong and Changchun Tu},
  journal={Vaccine},
  year={2006},
  volume={24 49-50},
  pages={7175-80}
}
Research on epitope-based vaccines is a current focus in the development of new vaccines against classical swine fever virus (CSFV). The present study aimed to engineer a quadruple antigenic epitope peptide of the CSFV immunogen E2 glycoprotein by splice overlap extension (SOE) PCR, expressed in E. coli fused with glutathione S-transferase (GST), and named rGST-4E. Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis showed that purified rGST-4E had an excellent immunoreactivity… CONTINUE READING

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