Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation.

@article{Irvine2011QPCRIC,
  title={Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation.},
  author={Ryan A. Irvine and Cindy Yen Okitsu and Chih-lin Hsieh},
  journal={Methods in molecular biology},
  year={2011},
  volume={791},
  pages={213-23}
}
Quantitative polymerase chain reaction (Q-PCR) allows for the accurate and reproducible determination of the amount of target DNA in a sample through the measurement of PCR product accumulation in "real time." This method determines starting target DNA quantity over a large assay dynamic range and requires no post-PCR sample manipulation. When used in combination with the method of chromatin immunoprecipitation (ChIP), the amount of protein binding to a specific region of DNA can be accurately… CONTINUE READING