Pyruvate shuttle in muscle cells: high-affinity pyruvate transport sites insensitive to trans-lactate efflux.

  title={Pyruvate shuttle in muscle cells: high-affinity pyruvate transport sites insensitive to trans-lactate efflux.},
  author={Raymond Mengual and K el Abida and N. Mouaffak and Michel Rieu and Mich{\`e}le Beaudry},
  journal={American journal of physiology. Endocrinology and metabolism},
  volume={285 6},
The specificity of the transport mechanisms for pyruvate and lactate and their sensitivity to inhibitors were studied in L6 skeletal muscle cells. Trans- and cis-lactate effects on pyruvate transport kinetic parameters were examined. Pyruvate and lactate were transported by a multisite carrier system, i.e., by two families of sites, one with low affinity and high capacity (type I sites) and the other with high affinity and low capacity (type II). The multisite character of transport kinetics… Expand
This work has concluded that exercise induced acidosis may not be deleterious to exercise performance and both [HCO3 - ] and [H + ] are dependant variables and therefore unable to be primarily or independently moderated. Expand
Einfluss des Transkriptionsfaktors Nuclear factor E2-related factor 2 (Nrf2) auf die Metabolische Reprogrammierung kolorektaler Zellen während der entzündlichen Karzinogenese des Darmes
Die Ar­beiten umfassten hierzu auf Zellkultur basierte Untersuchungen zur Rolle von Nrf2 bei der metabo­lischen Repro­grammierung, wie sich ein proinflammatorisches microenvironment auf pramaligne Kolonepithelzellen auswirkt werden. Expand


Lactate transport in L6 skeletal muscle cells and vesicles: allosteric or multisite mechanism and functional membrane marker of differentiation.
Membrane lactate transport was studied in skeletal muscle cells and membrane vesicles from the L6 line in relation to in vitro myogenesis. In myoblasts, lactate was transported by simple diffusionExpand
Kinetics of lactate and pyruvate transport in cultured rat myotubes.
Skeletal muscle transport of lactate and pyruvate was studied in primary cultures of rat myotubes, applying the pH-sensitive fluorescent indicator 2', 7'-bis(carboxyethyl)-5(6)- carboxyfluorescein, to determine total transport (carrier mediated and diffusion). Expand
Lactate Transport by Cortical Synaptosomes from Adult Rat Brain: Characterization of Kinetics and Inhibitor Specificity
The studies show that synaptic terminals from adult brain have a high capacity for transport and oxidation of lactate, consistent with the proposed role for this compound in metabolic trafficking in brain. Expand
Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle.
Lactate is the predominant monocarboxylate oxidized by mitochondria in vivo, and Mammalian liver and striated muscle mitochondria can oxidize exogenous lactate because of an internal LDH pool that facilitates lactate oxidation. Expand
Kinetic asymmetry of renal Na+-L-lactate cotransport. Characteristic parameters and evidence for a ping pong mechanism of the trans-stimulating exchange by pyruvate.
Kinetic parameters obtained have shown that under sodium lactate chemical gradient, the carrier efficiency is higher for the influx than efflux, a mechanism indicating a kinetic asymmetry of the transport, and a ping pong mechanism for the trans-activation exchange is demonstrated. Expand
Trans-stimulation of lactate transport from rat sarcolemmal membrane vesicles.
It is concluded that the sarcolemmal lactate transporter is stereospecific for L(+)-lactate; pyruvate can share the transporter, although the affinity of the transporter for pyruVate is less than that for L (+)- lactate. Expand
Current aspects of lactate exchange: lactate/H+ transport in human skeletal muscle
  • C. Juel
  • Chemistry, Medicine
  • European Journal of Applied Physiology
  • 2001
MCT1 and MCT4 respond differently to a high-intensity training session, which suggests that these two isoforms are regulated differently, and may have different roles in muscle. Expand
Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle
The characterisation of MCT4 expressed in Xenopus oocytes shows that the protein was correctly targeted to the plasma membrane and rates of substrate transport were determined from the rate of intracellular acidification monitored with the pH‐sensitive dye 2′,7′‐bis‐( carboxyethyl)‐5(6)‐carboxyfluorescein (BCECF). Expand
Lactate shuttles in nature.
  • G. Brooks
  • Biology, Medicine
  • Biochemical Society transactions
  • 2002
"Cell-cell" and "intracellular lactate shuttle" concepts describe the roles of lactate in the delivery of oxidative and gluconeogenic substrates, as well as in cell signalling, and give rise to the notion that glycolytic and oxidative pathways can be viewed as linked, as opposed to alternative, processes. Expand
The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.
There is still much work to be done to characterize the properties of the different MCT isoforms and their regulation, which may have wide-ranging implications for health and disease. Expand