Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens

  title={Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens},
  author={Shadi Shokralla and Xin Zhou and Daniel H Janzen and Winnie Hallwachs and Jean-François Landry and Luke M. Jacobus and Mehrdad Hajibabaei},
  booktitle={PloS one},
DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficult to obtain a full-length barcode from old specimens. Mini-barcodes of 130 bp from the standard barcode region have been shown to be effective for accurate identification in many animal groups and… CONTINUE READING