Pyrophosphate of high and low energy. Contributions of pH, Ca2+, Mg2+, and water to free energy of hydrolysis.

@article{Meis1984PyrophosphateOH,
  title={Pyrophosphate of high and low energy. Contributions of pH, Ca2+, Mg2+, and water to free energy of hydrolysis.},
  author={Leopoldo de Meis},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 10},
  pages={6090-7}
}
The equilibrium between inorganic pyrophosphate and inorganic orthophosphate was determined at pH values varying between 6.0 and 8.0, in the presence of different concentrations of MgCl2, mixtures of MgCl2 and CaCl2, and different organic solvents. The reactions were catalyzed by yeast inorganic pyrophosphatase. It was found that at 35 degrees C, depending on the conditions used, the observed equilibrium constant of pyrophosphate hydrolysis vary from a value higher than 4 X 10(3) M (delta Goobs… CONTINUE READING

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