Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.

@article{Kottur2018PyrophosphateHI,
  title={Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.},
  author={Jithesh Kottur and Deepak T Nair},
  journal={Nucleic acids research},
  year={2018},
  volume={46 12},
  pages={
          5875-5885
        }
}
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release of a pyrophosphate (PPi) group. It is believed that formation of the… CONTINUE READING