Pyrophosphate formation from acetyl phosphate and orthophosphate: evidence for heterogeneous catalysis.

@article{MeyerFernandes1988PyrophosphateFF,
  title={Pyrophosphate formation from acetyl phosphate and orthophosphate: evidence for heterogeneous catalysis.},
  author={Jos{\'e} Roberto Meyer-Fernandes and Adalberto Vieyra},
  journal={Archives of biochemistry and biophysics},
  year={1988},
  volume={266 1},
  pages={132-41}
}
The formation of [32P]pyrophosphate from acetyl phosphate and [32P]orthophosphate was studied under conditions in which phosphate-metal salts or acetyl phosphate-metal salts precipitate. In the absence of precipitates in purely aqueous media, the initial rate constant of transphosphorylation (kobs) was extremely small and the formation of pyrophosphate was detected only in the presence of calcium. In various combinations, conditions such as high pH, high concentrations of reactants, and the… CONTINUE READING