Pyrophosphatase is essential for growth of Escherichia coli.

  title={Pyrophosphatase is essential for growth of Escherichia coli.},
  author={Josiane Chen and Annie Brevet and Michel Fromant and François L{\'e}v{\^e}que and Jean Marie Schmitter and Sylvain Blanquet and Pierre Plateau},
  journal={Journal of bacteriology},
  volume={172 10},
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular… CONTINUE READING

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