PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data

@inproceedings{Weissbrodt2012PyroTRFIDAN,
  title={PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data},
  author={David G Weissbrodt and Noam Shani and Lucas Sinclair and Gregory Lefebvre and Pierre Rossi and Julien Maillard and Jacques Rougemont and Christof Holliger},
  booktitle={BMC Microbiology},
  year={2012}
}
In molecular microbial ecology, massive sequencing is gradually replacing classical fingerprinting techniques such as terminal-restriction fragment length polymorphism (T-RFLP) combined with cloning-sequencing for the characterization of microbiomes. Here, a bioinformatics methodology for pyrosequencing-based T-RF identification (PyroTRF-ID) was developed… CONTINUE READING