Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR

@article{Huang1997PyramidingOB,
  title={Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR},
  author={Ning Huang and Enrique R. Angeles and Javier D{\'i}ez Domingo and Gerard B. Magpantay and Sukhwinder Singh and Guiquan Zhang and N. Kumaravadivel and John F. Bennett and Gurdev Singh Khush},
  journal={Theoretical and Applied Genetics},
  year={1997},
  volume={95},
  pages={313-320}
}
 DNA marker-assisted selection was used to pyramid four bacterial blight resistance genes, Xa-4, xa-5, xa-13 and Xa-21. Breeding lines with two, three and four resistance genes were developed and tested for resistance to the bacterial blight pathogen (Xanthomonas oryzae pv. oryzae). The pyramid lines showed a wider spectrum and a higher level of resistance than lines with only a single gene. To speed up the gene pyramiding process and to facilitate future marker-aided selection, we developed… CONTINUE READING
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