Pyocin typing of Pseudomonas aeruginosa: a simplified method.

  title={Pyocin typing of Pseudomonas aeruginosa: a simplified method.},
  author={Lawrence W. Jones and J P Zakanycz and Ed. Thomas and Jim J Farmer},
  journal={Applied microbiology},
  volume={27 2},
A simplified method has been devised for typing Pseudomonas aeruginosa by pyocin production. Pyocins are produced as strains grow overnight in Trypticase soy broth (without glucose) plus 1% potassium nitrate. Because P. aeruginosa can use nitrate instead of oxygen as a terminal electron acceptor, mechanical shaking is not necessary, nor is induction by mitomycin C. Pyocins can now be produced in screw-cap tubes in a water bath or incubator. A total of 250 strains were tested as possible pyocin… CONTINUE READING

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