Putative metabolic pathway for the bioproduction of bikaverin and intermediates thereof in the wild Fusarium oxysporum LCP531 strain

  title={Putative metabolic pathway for the bioproduction of bikaverin and intermediates thereof in the wild Fusarium oxysporum LCP531 strain},
  author={Juliana Lebeau and Thomas J.P. Petit and Laurent Dufoss{\'e} and Yanis Caro},
  journal={AMB Express},
Fungal naphthoquinones, like red bikaverin, are of interest due to their growing applications in designing pharmaceutical products. Though considerable work has been done on the elucidation of bikaverin biosynthesis pathway in Fusarium fujikuroi, very few reports are available regarding its bioproduction in F. oxysporum. We are hereby proposing a putative metabolic pathway for bikaverin bioproduction in a wild F. oxysporum strain by cross-linking the pigment profiles we obtained under two… 
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Redirection of pigment biosynthesis to isocoumarins in Fusarium.
Isolation of two novel purple naphthoquinone pigments concomitant with the bioactive red bikaverin and derivates thereof produced by Fusarium oxysporum
This is the first report of these two novel wild‐type purple naphthoquinones pigments along with bikaverin, where additionally, the culture conditions were put into perspective to optimize fermentation cultures and extraction process accordingly to the pigment/biomolecule desired.
Biosynthesis of the red pigment bikaverin in Fusarium fujikuroi: genes, their function and regulation
The characterization of five genes adjacent to bik1 as encoding a putative FAD‐dependent monooxygenase (bik2), an O‐methyltransferase (Bik3), an NmrA‐like protein ( bik4), a Zn(II)2Cys6 transcription factor and an MFS transporter (bk6) results in total loss or significant reduction of bikaverin synthesis.
Bikaverin production and applications
The diverse biological activities and the increasing information on the biochemical and genetic basis of its production make bikaverin a metabolite of increasing biotechnological interest.
A Functional Bikaverin Biosynthesis Gene Cluster in Rare Strains of Botrytis cinerea Is Positively Controlled by VELVET
Evidence is provided that rare B. cinerea strains such as 1750 have a complete and functional cluster comprising the six genes orthologous to Fusarium fujikuroi ffbik1-ffbik6 and do produce bikaverin.
Stimulation of bikaverin production by sucrose and by salt starvation in Fusarium fujikuroi
The induction by salt starvation, but not by sucrose, correlated with an increase in messenger RNA levels of gene bik1, encoding a polyketide synthase of the bikaverin pathway, an effect not exhibited for the production of gibberellins and carotenoids.
Bikaverin production by Fusarium species
The production of bikaverin determines the colour of the mentionedFusarium species cultures on agar media and on rice and the role it plays in fungus metabolism is not elucidated.
Identification and Regulation of fusA, the Polyketide Synthase Gene Responsible for Fusarin Production in Fusarium fujikuroi
Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins, and are less efficient than the wild type at degrading cellophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum.
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chemical and X-ray crystallographic evidence established the structure of bikaverin as I. lycopersici, a fungal vacuolation factor discovered by Park and coworkers in aging cultures of F. oxysporum and F. sp.