Putative markers for the detection of breast carcinoma cells in blood.

  title={Putative markers for the detection of breast carcinoma cells in blood.},
  author={Elrashid M Eltahir and Dominic Mallinson and George D. Birnie and Christy Hagan and W. David George and Anand David Purushotham},
  journal={British Journal of Cancer},
  pages={1203 - 1207}
The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin 19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1… CONTINUE READING


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