Putative GalP, XylE, and FucP H+ symporters from Pantoea ananatis are capable of transporting glucose into Escherichia coli cells

@article{Andreeva2013PutativeGX,
  title={Putative GalP, XylE, and FucP H+ symporters from Pantoea ananatis are capable of transporting glucose into Escherichia coli cells},
  author={Irina G. Andreeva and Lyubov I. Golubeva and Joanna I. Katashkina},
  journal={Applied Biochemistry and Microbiology},
  year={2013},
  volume={49},
  pages={638-645}
}
The involvement of presumably low-affinity H+ symporter GalP in the glucose uptake by Pantoea ananatis cells was demonstrated. The putative galP, xylE, and fucP genes from P. ananatis AJ13355—orthologs of the known E. coli genes for H+ symporters of D-galactose, D-xylose, and L-fucose, respectively, were cloned. It was confirmed that the constitutive expression of each of the cloned genes restored the deleted E. coli MG1655Δ(ptsHI-crr) strain growth on D-glucose. The constructed integrative… CONTINUE READING

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