Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.

@article{Vogt2014PushingPF,
  title={Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.},
  author={Michael Vogt and Sabine Haas and Simon Klaffl and Tino Polen and Lothar Eggeling and Jan van Ooyen and Michael Bott},
  journal={Metabolic engineering},
  year={2014},
  volume={22},
  pages={40-52}
}
Using metabolic engineering, an efficient L-leucine production strain of Corynebacterium glutamicum was developed. In the wild type of C. glutamicum, the leuA-encoded 2-isopropylmalate synthase (IPMS) is inhibited by low L-leucine concentrations with a K(i) of 0.4 mM. We identified a feedback-resistant IMPS variant, which carries two amino acid exchanges (R529H, G532D). The corresponding leuA(fbr) gene devoid of the attenuator region and under control of a strong promoter was integrated in one… CONTINUE READING
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